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Published on: May 4, 2018
The NII2 gene of Hansenula polymorpha is involved in nitrite assimilation
Federica Serrani1, Enrico Berardi
1Laboratorio di Genetica Microbica, DiSA, Università Politecnica delle Marche, Via Brecce Bianche, 60131 Ancona, Italy.
Abstract:
To establish a basis for genetic and molecular studies of nitrite assimilation in the methylotrophic yeast Hansenula polymorpha, we isolated and characterised six nitrite-negative mutants still capable of growing on nitrate. Gene isolation work yielded the NII2 gene, encoding a membrane protein homologous to the Saccharomyces cerevisiae Pho86p. Sequence analysis revealed an ORF of 860 bp encoding a 286-amino-acid protein with a predicted molecular mass of 32.8 kDa. This protein is shorter than its S. cerevisiae homologue, and is predicted to lack an ER-retention signal. Cell suspension work revealed that the null mutant is unable to take up nitrite from the medium.
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