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Multilocus sequence typing of intercontinental bovine Staphylococcus aureus isolates.

E M Smith1, L E Green, G F Medley

  • 1Department of Biological Sciences, University of Warwick, Coventry, UK.

Journal of Clinical Microbiology
|September 8, 2005
PubMed
Summary

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Multilocus sequence typing (MLST) effectively differentiates Staphylococcus aureus isolates from cattle across different countries and sample types. A dominant clonal complex suggests a widespread, host-specific bacterial group responsible for most bovine infections.

Area of Science:

  • Veterinary Microbiology
  • Bacterial Genomics

Background:

  • Staphylococcus aureus is a significant pathogen in cattle, causing mastitis and other infections.
  • Understanding the genetic diversity and population structure of bovine S. aureus is crucial for effective control strategies.

Purpose of the Study:

  • To evaluate the suitability of multilocus sequence typing (MLST) for characterizing bovine-associated Staphylococcus aureus.
  • To investigate the population structure and host specificity of S. aureus isolates from cattle in different geographic regions.

Main Methods:

  • Analysis of 258 bovine-associated Staphylococcus aureus isolates using multilocus sequence typing (MLST).
  • Inclusion of isolates from the United States, Chile, and the United Kingdom, alongside a reference strain.
  • Comparison with previously characterized United Kingdom isolates.

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Main Results:

  • MLST proved effective in differentiating bovine S. aureus isolates from various sample sites (milk, teat skin, equipment, hands, bedding) and countries.
  • A novel clonal complex, comprising 87.4% of isolates from all locations, was identified, supporting the theory of host specificity.
  • Specific sequence types (ST25, ST115, ST124, ST126) showed significant association with milk or teat skin, indicating site specificity.

Conclusions:

  • MLST is a valuable tool for studying the epidemiology of bovine Staphylococcus aureus.
  • A dominant, widespread clonal group of Staphylococcus aureus appears highly adapted to cattle, responsible for the majority of infections.
  • Evidence suggests specific sequence types may have tropism for particular bovine anatomical sites.