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Published on: March 8, 2012
Comparison of three different PCR methods for quantifying human papillomavirus type 16 DNA in cervical scrape
A T Hesselink1, A J C van den Brule, Z M A Groothuismink
1Department of Pathology, VU University Medical Center, Amsterdam, The Netherlands.
Journal of Clinical Microbiology
|September 8, 2005
Abstract:
We compared real-time LightCycler and TaqMan assays and the GP5+/6+ PCR/enzyme immunoassay (EIA) to assess the human papillomavirus type 16 (HPV16) load in cervical scrape specimens. Both real-time PCR assays determined the HPV16 load in scrape specimens similarly. The level of agreement between these assays and the GP5+/6+ PCR/EIA was low (P = 0.004), suggesting that the latter method is not suited for quantifying HPV16 DNA.
