Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A method for exceptionally low noise single channel recordings.

J L Rae1, R A Levis

  • 1Department of Physiology and Biophysics, Mayo Foundation, Rochester, MN 55905.

Pflugers Archiv : European Journal of Physiology
|April 1, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Patch Clamp Recordings from the Epithelium of the Lens Obtained using Glasses Selected for Low Noise and Improved Sealing Properties.

Biophysical journal·2009
Same author

Fabrication of patch pipets.

Current protocols in neuroscience·2008
Same author

SCN5A is expressed in human jejunal circular smooth muscle cells.

Neurogastroenterology and motility·2002
Same author

Kv3.3 potassium channels in lens epithelium and corneal endothelium.

Experimental eye research·2000
Same author

Kir2.1 Potassium channels and corneal epithelia.

Current eye research·2000
Same author

A mechanosensitive calcium channel in human intestinal smooth muscle cells.

Gastroenterology·1999

Researchers developed a new method for single-channel patch clamp recordings, significantly reducing background noise. This advancement improves signal-to-noise ratios, enabling clearer data acquisition in electrophysiology research.

Area of Science:

  • Electrophysiology
  • Biophysical Techniques
  • Neuroscience Instrumentation

Background:

  • Patch clamp recording is a crucial technique for studying ion channels.
  • High background noise limits signal-to-noise ratios and restricts recording bandwidth.
  • Previous methods have not achieved the current low noise levels.

Purpose of the Study:

  • To present a novel method for reducing background noise in single-channel patch clamp recordings.
  • To enhance signal-to-noise ratios for improved electrophysiological data.
  • To enable researchers to increase recording bandwidths or maintain low noise levels.

Main Methods:

  • Integration of specialized electronics.
  • Utilization of quartz patch electrodes.

Related Experiment Videos

  • Novel application of silicone oil to minimize noise interference.

    • Main Results:

    • Achieved exceptionally low background noise levels of 0.083 pA RMS.
    • Recordings were performed within a 5 kHz bandwidth using a 4-pole Butterworth filter.
    • Demonstrated significantly higher signal-to-noise ratios compared to previously reported methods.

    Conclusions:

    • The presented method offers a substantial improvement in noise reduction for patch clamp recordings.
    • This technique allows for increased recording bandwidths or maintained low noise at constant bandwidths.
    • Facilitates more accurate and detailed electrophysiological studies across various research fields.