Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Molecular engineering approaches for DNA sequencing and analysis.

Xiaopeng Bai1, John Edwards, Jingyue Ju

  • 1Columbia University, Department of Chemical Engineering, NY 10027, USA. xb8@columbia.edu

Expert Review of Molecular Diagnostics
|September 10, 2005
PubMed
Summary

Advancements in high-throughput DNA sequencing are crucial for pharmacogenomics and personalized medicine. This review explores two key technologies: molecular affinity/mass spectrometry and sequencing-by-synthesis for mutation detection.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Rhabdomyolysis-Induced Resistant Hypercalcemia During the Recovery Phase of Acute Kidney Injury.

Cureus·2026
Same author

RNA with Azvudine Incorporated One Nucleotide Upstream of 3'-End Resists Cleavage by SARS-CoV-2 Proofreading Exonuclease.

Research square·2026
Same author

Harmonized <sup>1</sup>H NMR Workflow Enables Quantitative Betaine Determination from Nontargeted Metabolite Profiling Using Internal and External Standards<sup>‡‡‡‡‡</sup>.

Analytical chemistry·2026
Same author

Design and Experimental Verification of a Gibbon-Inspired Tree-Climbing Robot for Forestry Environments.

Biomimetics (Basel, Switzerland)·2026
Same author

Zinc excess promotes lysosome remodeling by activating HLH-30/TFEB through the action of the high zinc sensor HIZR-1.

bioRxiv : the preprint server for biology·2026
Same author

Esophageal Hyposensitivity: A Key Link to Aspiration Pneumonia in Patients with Achalasia.

Digestion·2026

Area of Science:

  • Genomics and Molecular Biology
  • Biotechnology and Bioengineering
  • Clinical Medicine and Healthcare

Background:

  • High-throughput DNA sequencing is fundamental for advancing pharmacogenomics and personalized medicine.
  • Molecular engineering of DNA building blocks enables novel high-throughput genetic analysis technologies.
  • Current healthcare paradigms are shifting towards data-driven, individualized treatment strategies.

Purpose of the Study:

  • To review two cutting-edge DNA sequencing approaches for mutation screening and identification.
  • To discuss the principles, challenges, and implementation methods of these advanced technologies.
  • To highlight the potential impact on clinical medicine and personalized healthcare.

Main Methods:

  • Review of molecular affinity and mass spectrometry for rapid, accurate mutation screening.

Related Experiment Videos

  • Analysis of sequencing-by-synthesis platforms for massive, parallel DNA sequencing.
  • Discussion of molecular engineering strategies for DNA purification and detection.

    • Main Results:

    • Molecular affinity and mass spectrometry enable efficient heterozygote identification and indel detection.
    • Sequencing-by-synthesis platforms offer immense potential for large-scale DNA sequencing data generation.
    • These technologies represent significant progress in genetic analysis capabilities.

    Conclusions:

    • Developed DNA sequencing technologies are essential for the realization of pharmacogenomics.
    • These advancements pave the way for a new era in personalized medicine and clinical healthcare.
    • Further exploration of principles and challenges will optimize implementation of these powerful tools.