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Related Experiment Videos

cis-inhibitory elements in the pT181 replication system.

S J Projan1, R P Novick

  • 1Department of Applied Genetics, Public Health Research Institute, New York, New York 10016.

Plasmid
|March 1, 1992
PubMed
Summary
This summary is machine-generated.

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Two sequence elements in plasmids inhibit pT181 replication origins. This cis-inhibition is relieved by deleting specific elements or promoters, suggesting a post-binding inhibition step in replication initiation.

Area of Science:

  • Molecular Biology
  • Plasmid Biology
  • Replication Mechanisms

Background:

  • The pT181 replication system is a model for studying plasmid DNA replication.
  • Plasmid cointegrates can exhibit complex regulatory interactions affecting replication.
  • Understanding cis-acting elements is crucial for controlling plasmid replication.

Purpose of the Study:

  • To identify and characterize sequence elements that inhibit pT181 replication origins in cis.
  • To elucidate the mechanism of cis-inhibition of plasmid replication.
  • To investigate the role of specific plasmid elements in regulating replication initiation.

Main Methods:

  • Cloning of pT181 replication origins into the pE194 vector.
  • Construction and analysis of various plasmid cointegrates with deletions or modifications.

Related Experiment Videos

  • Assessment of replication origin function and cis-inhibition effects.
  • Analysis of plasmid incompatibility to determine the stage of inhibition.

    • Main Results:

    • Isolated pT181 replication origins are functional in pE194, but inactive in the full pT181 genome context.
    • Cis-inhibition is relieved by deleting the pE194 palA element or the pT181 countertranscript promoter.
    • The pE194 palA element's inhibitory effect is orientation and distance-independent.
    • The pT181 countertranscript promoter's inhibition is orientation and distance-dependent.
    • The cis-inhibited origin exhibits Inc3B incompatibility, indicating initiator protein competition.

    Conclusions:

    • A pair of sequence elements in plasmid cointegrates can inhibit pT181 replication origins in cis.
    • Inhibition affects a step in replication initiation subsequent to initiator protein binding.
    • Plasmid elements can act in cis to regulate replication, providing insights into replication control mechanisms.