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Nitrogenase switch-off by ammonium ions in Azospirillum brasilense requires the GlnB nitrogen signal-transducing
Giseli Klassen1, Emanuel M Souza, M Geoffrey Yates
1Universidade Federal do Paraná, Departamento de Bioquímica e Biologia Molecular, Caixa Postal 19046 CEP-81531-990, Curitiba, Paraná, Brazil.
Abstract:
Nitrogenase activity in several diazotrophs is switched off by ammonium and reactivated after consumption. The signaling pathway to this system in Azospirillum brasilense is not understood. We show that ammonium-dependent switch-off through ADP-ribosylation of Fe protein was partial in a glnB mutant of A. brasilense but absent in a glnB glnZ double mutant. Triggering of inactivation by anaerobic conditions was not affected in either mutant. The results suggest that glnB is necessary for full ammonium-dependent nitrogenase switch-off in A. brasilense.
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