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Updated: Aug 16, 2026

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
Published on: September 10, 2014
Determination of biochemical species on electrophoresis chips with an external contactless conductivity detector
Eva M Abad-Villar1, Pavel Kubán, Peter C Hauser
1Department of Chemistry, University of Basel, Basel, Switzerland.
Abstract:
Contactless conductivity measurements were found to be suitable for the direct detection, i.e., without needing any labels, of a range of biochemically relevant species, namely amino acids, peptides, proteins, immunoglobulin, and DNA. It was also possible to monitor the products of the enzymatic digestion of HSA with pepsin. Detection was carried out on bare electrophoresis chips made from poly(methyl methacrylate) by probing the conductivity in the channel with a pair of external electrodes, which are fixed on the chip holder. Separation efficiencies up to 15,000 plates could be obtained and LODs are in the low muM-range, except for immunoglobulin G (IgG) which could be determined down to 0.4 nM. Linear dynamic ranges of two to three orders of magnitude were obtained for the peptides as examples.
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