Upper and lower bounds for a serial dilution test
Related Experiment Video
Updated: Nov 7, 2025
07:43
Author Spotlight: Addressing Regulatory Gaps in Molecular Studies by Quantifying Viral Vectors in Complex Matrices
Published on: July 14, 2023
2.6K
1U.S. Food and Drug Administration, HFS-705, Rm 2D-011, 5100 Paint Branch Pkwy, College Park, MD 20740, USA. Robert.Blodgett@cfsan.fda.gov
Journal of AOAC International
|September 13, 2005
Abstract:
A Poisson-binomial model estimates the concentration of a target microbe from a serial dilution test. The maximum likelihood procedure gives an equation whose solution equals the estimate of the concentration. This paper gives bounds for the solution to this equation that require only minimal calculations.
Related Concept Videos
01:23Microbial Growth Measurement: Direct Methods
950
Direct methods for measuring microbial populations in a culture are essential tools in microbiology, providing quantitative data for various applications. Among these, microscopic counts, plate counts, and serial dilution are widely used techniques, each with unique principles and applications.Microscopic CountsMicroscopic counting involves the use of a Petroff-Hausser chamber, a specialized microscope slide with a grid and defined depth. By observing a liquid culture under a microscope,...
950
01:05Difference from Background: Limit of Detection
7.5K
The limit of detection (LOD) is the smallest amount of analyte that can be distinguished from the background noise. The LOD value corresponds to the concentration at which the analyte signal is three times larger than the standard deviation of the blank signal. Below this value, the analyte signal cannot be differentiated from the background noise. It is calculated by dividing the calibration slope by 3 times the standard deviation of the blank signals.
The LOD indicates the presence or absence...
The LOD indicates the presence or absence...
7.5K