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Zingerone as an antioxidant against peroxynitrite
Sang-Guk Shin1, Ji Young Kim, Hae Young Chung
1Department of Internal Medicine, College of Oriental Medicine, Dong-Guk University, Pusan National University, Busan, Korea.
Journal of Agricultural and Food Chemistry
|September 15, 2005
Summary
Zingerone, a compound from ginger, effectively neutralizes harmful peroxynitrite (ONOO(-)) and reactive species. This antioxidant activity suggests zingerone could protect against diseases linked to peroxynitrite-induced cellular damage.
Area of Science:
- Biochemistry
- Pharmacology
- Oxidative Stress Research
Background:
- Peroxynitrite (ONOO(-)) formation from superoxide and nitric oxide causes significant cellular and tissue injury.
- This injury is implicated in various human diseases, including stroke, Alzheimer's disease, and atherosclerosis.
- The absence of endogenous enzymes for ONOO(-) scavenging highlights the need for external ONOO(-) scavengers.
Purpose of the Study:
- To investigate the peroxynitrite (ONOO(-)) scavenging capabilities of zingerone, a compound derived from ginger.
- To evaluate zingerone's efficacy against both native ONOO(-) and intracellular reactive species.
Main Methods:
- Assessing zingerone's direct scavenging of ONOO(-).
- Testing zingerone's ability to scavenge ONOO(-) generated by SIN-1.
- Evaluating zingerone's inhibition of ONOO(-)-mediated tyrosine nitration and BSA nitration.
- Measuring the scavenging of intracellular reactive species and ONOO(-) by zingerone.
Main Results:
- Zingerone demonstrated efficient scavenging of native peroxynitrite (ONOO(-)).
- Zingerone effectively scavenged ONOO(-) generated from the donor SIN-1.
- Zingerone inhibited ONOO(-)-induced tyrosine nitration via electron donation.
- Zingerone reduced intracellular reactive species and ONOO(-) levels.
Conclusions:
- Zingerone exhibits significant peroxynitrite (ONOO(-)) scavenging activity.
- The findings suggest zingerone is a potent scavenger for protecting against ONOO(-)-involved diseases.
- Zingerone's antioxidant properties may offer cellular defense against ONOO(-)-induced damage.