Related Experiment Video
Updated: Aug 16, 2026
Discovery of Driver Genes in Colorectal HT29-derived Cancer Stem-Like Tumorspheres
Published on: July 22, 2020
[Effect of NGX6 on gene expression profile of colon carcinoma cell line HT-29]
Fen Liu1, Xiao-Yan Wang, Ping Lian
1Department of Digestion Medicine, The Third Xiangya Hospital, Central South University, Changsha, Hunan, P.R. China.
Background & Objective:
Colon cancer is one of the most common malignant tumors, but the mechanism involved in its pathogenesis is not fully understood. Under the foundation of early study, we intended to explore the effects of NGX6 on the gene expression profile of colon carcinoma cell line HT-29.
Methods:
pcDNA3.1-NGX6-transfected HT-29 cells were used as the test, and empty vector-transfected HT-29 cells were used as the control. Differentially expressed genes were screened with high-throughout BiostarH-80sx1 DNA microarray; part of the results of DNA microarray was confirmed by reverse transcription-polymerase chain reaction (RT-PCR).
Results:
NGX6 transfection induced wide changes of the gene expression profile of HT-29 cells. A total of 377 genes were up-regulated or down-regulated by more than 3 folds, which were involved in cell signal transduction, cell cycle controlling, cell apoptosis, cytokinetics, gene transcription and translation, DNA damage and repairing, protein synthesis, metabolism, and so on. Some of them belonged to some important signal pathways related with cell proliferation and metastasis, such as DDK1, ILK, MMP-1 and COL11A1 in Wnt/beta-catenin signal pathway, ILK in ILK signal pathway, EpHB2 in Eph-Ephrins signal pathway, ROCK2 in RhoA signal pathway, RANBP1 in RanGTPase signal pathway, and RBBP1 in RB/RBBP1 signal pathway.
Conclusion:
NGX6 transfection leads to molecular changes of some important signal pathways, and may suppress cell proliferation and metastasis of tumor by regulating these signal pathways.
Insights
NGX6 gene expression changes in colon cancer cells HT-29 were identified. These alterations in signaling pathways may inhibit tumor cell proliferation and metastasis.
Area of Science:
- Molecular Biology
- Genomics
- Cancer Research
Context:
- Colon cancer pathogenesis remains incompletely understood.
- Investigating novel molecular mechanisms is crucial for therapeutic development.
Purpose:
- To explore the effects of NGX6 on the gene expression profile of the colon carcinoma cell line HT-29.
- To identify specific genes and pathways regulated by NGX6.
Summary:
- NGX6 transfection in HT-29 cells induced significant changes in gene expression, with 377 genes (3-fold change) identified.
- Affected genes are involved in critical cellular processes including signal transduction, cell cycle, apoptosis, and DNA repair.
- NGX6 impacts key signaling pathways such as Wnt/beta-catenin, ILK, Eph-Ephrins, RhoA, RanGTPase, and RB/RBBP1, which are implicated in tumor progression.
Impact:
- NGX6 influences multiple signaling pathways, suggesting a potential role in regulating colon cancer cell proliferation and metastasis.
- This study provides a foundation for understanding NGX6's function and its therapeutic potential in colon cancer.
