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Study on the Metabolism of Six Systemic Insecticides in a Newly Established Cell Suspension Culture Derived from Tea (Camellia Sinensis L.) Leaves
Published on: June 15, 2019
[Cell suspension culture and flavonoids production in Saussurea involucrate]
Li-qin Wu1, Shun-xing Guo, Pei-gen Xiao
1Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100094, China.
Objective:
To establish a system of cell suspension culture for Saussurea involucrate.
Method:
The effects of different factors on cell growth and flavonoids production of S. involucrate were systematically studied including the media, initial pH values of the medium, carbon sources, inoculum quantity, and plant growth substance.
Result:
The optimum medium was N6, initial pH values of the medium was 5.8, sucrose concentration was 50 g x L(-1), inoculum quantity was 60-80 g x L(-1) FW. Medium supplemented with BA (0.5 mg x L(-1)) and NAA (3 mg x L(-1)) was suitable for cell growth, but medium containing BA (0.2 mg x L(-1)) and NAA (2 mg x L(-1)) was suitable for flavonoids production.
Conclusion:
Cell growth and flavonoids production in the suspention of S. involucrate culture cell should be optimized.
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