Related Experiment Video
Updated: Aug 16, 2026
A Freeze-Thawing Method to Prepare Chitosan-Poly(vinyl alcohol) Hydrogels Without Crosslinking Agents and Diflunisal Release Studies
Published on: January 14, 2020
[Preparation and quality control of low molecular weight chitosan]
Xuan Huang1, Fu-Qiang Hu, Hong Yuan
1College of Pharmaceutical Scione, Zhejiang University, Hangzhou 310031, China.
Objective:
To prepare the low molecular weight chitosan (LMWC) and establish the method for quality control.
Method:
Use enzymatic degradation to prepare LMWC with chitosan, and separate by ultrafiltration; the molecular weight and purity were determined by gel permeation chromatography (GPC) and colorimetry respectively.
Result:
LMWC was prepared by control the hours of enzymatic degradation and ultrafiltered through filter with cutoff molecular of 10K Dalton and 50 K dalton; the average molecular weight was 20 K dalton and the purity was (96.60 +/- 1.56)%.
Conclusion:
The condition of enzymatic degradation is geniality and easy to control, LMWCs with different molecular weight can separate by ultrafiltration efficiently; the quality of LMWC can control with gel permeation chromatography (GPC) and colorimetry.
