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Updated: Aug 15, 2026
An Integrated System to Remotely Trigger Intracellular Signal Transduction by Upconversion Nanoparticle-mediated Kinase Photoactivation
Published on: August 30, 2017
Optical nanoinjection of macromolecules into vital cells
Frank Stracke1, Iris Rieman, Karsten König
1Fraunhofer Institute for Biomedical Technology (IBMT), Lasermedicine, Ensheimer Street 48, D-66386 St. Ingbert, Germany. frank.stracke@ibmt.fraunhofer.de
Abstract:
A tuneable near infrared 90MHz mode locked femtosecond laser was applied for targeted multiphoton optoporation of vital cells. Here, we demonstrate the laser assisted transfer of large amounts of exogenous materials and even macromolecules into living cells via a transient opening of the membrane and quantify the influx. The use of near infrared lasers also allow the optoporation of cells deep in three dimensional biological structures without photodestructive collateral effects.
