Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Oxidant stress suppresses CFTR expression.

André M Cantin1, Ginette Bilodeau, Cristine Ouellet

  • 1Pulmonary Research Unit, Faculty of Medicine, Univ. of Sherbrooke, 3001, 12ième Ave. Nord, Sherbrooke, QC, Canada J1H 5N4. Andre.Cantin@USherbrooke.ca

American Journal of Physiology. Cell Physiology
|September 16, 2005
PubMed
Summary

Oxidant stress reduces cystic fibrosis transmembrane conductance regulator (CFTR) expression in epithelial cells. This suppression of CFTR may be an adaptive response to protect mucosal epithelium from damage.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Integrated transcriptomic and proteomic analysis reveals the regulatory role of exogenous gibberellin in sugarcane internode maturation.

Frontiers in plant science·2026
Same author

Reduced left dorsolateral prefrontal activation and right inferior frontal de-oxygenation differ between psychotic and non-psychotic adolescent depression during verbal fluency.

Frontiers in psychiatry·2026
Same author

Low miR-223 links to major adverse cardiovascular and cerebrovascular events in end-stage renal disease through endothelial damage.

BMC nephrology·2026
Same author

Metabolomics Integrated with Mass Spectrometry Imaging Reveals Novel Action of Rb1 in Ischemic Stroke.

Journal of proteome research·2026
Same author

Measurement of Protein Transport in Heterogeneous Environments Using Confinement k-Space Image Correlation Spectroscopy.

Biomolecules·2026
Same author

Severe Hypoxia Reduces Adenylyl Cyclase 6 Expression and Function in Bronchial Epithelial Cells.

FASEB bioAdvances·2026

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Physiology

Background:

  • Epithelial mucous membranes face constant exposure to oxidants and xenobiotics.
  • Cystic fibrosis transmembrane conductance regulator (CFTR) influences glutathione transport and mucus properties.
  • The role of CFTR under oxidant stress conditions is not fully understood.

Purpose of the Study:

  • To investigate the hypothesis that CFTR expression and function are modulated by oxidant stress.
  • To determine the molecular mechanisms underlying changes in CFTR expression during oxidative stress.

Main Methods:

  • Exposure of T84 and Calu-3 epithelial cells to sublethal oxidant stress (tert-butylhydroquinone, BHQ) and taurine chloramine.
  • Measurement of cellular glutathione levels.

Related Experiment Videos

  • Analysis of gamma-glutamylcysteine synthetase heavy subunit (gamma-GCShs) and CFTR gene and protein expression using RT-PCR and Western blotting.
  • Assessment of CFTR function via cAMP-dependent iodide efflux assays.
  • Nuclear run-on assays to evaluate CFTR gene transcription and mRNA stability.

    • Main Results:

    • Oxidant stress significantly increased cellular glutathione and gamma-GCShs expression.
    • CFTR gene and protein expression were markedly decreased in response to oxidant stress.
    • CFTR function, measured by iodide efflux, was reduced under oxidant stress.
    • Oxidant stress decreased CFTR mRNA stability without affecting transcription.
    • Similar effects on gamma-GCShs and CFTR mRNA were observed in Calu-3 cells, and taurine chloramine induced comparable changes in T84 cells.

    Conclusions:

    • Suppression of CFTR expression is induced by exogenous oxidant stress in mucosal epithelial cells.
    • Reduced CFTR expression and function may represent an adaptive mechanism to protect the epithelium.
    • Oxidative stress impacts CFTR expression primarily through post-transcriptional regulation, specifically mRNA destabilization.