Related Experiment Video
Updated: Aug 15, 2026
Murine Lymphocyte Labeling by 64Cu-Antibody Receptor Targeting for In Vivo Cell Trafficking by PET/CT
Published on: April 29, 2017
[Lymph kinetics with technetium-99m labeled radiopharmaceuticals. Animal studies]
M Weiss1, F J Gildehaus, K Brinkbäumer
1Klinik und Poliklinik für Nuklearmedizin, Ludwig-Maximilians-Universität München, Ziemssenstrasse 1, 80335 München, Germany. mayo.weiss@med.uni-muenchen.de
Unlabelled:
Aim of this study was to characterize suitable technetium-99m labeled tracers for lymphoscintigraphy by comparative animal tests.
Animals, Methods:
To evaluate the influence of the particle size and the organ tracer-uptake on lymphtransport animal experiments were performed on six different agents (including one control group). Activity distributions were examined in Sprague-Dawley-rats by lymphoscintigraphy; the maximum uptake (count-rate) of the whole body and in lymph nodes were analyzed by regions-of-interest-technique, respectively. Additionally, for characterization of lymphatic and extralymphatic traceruptake an intra-individual relative count-rate ratio of the liver, lung, kidneys, and spleen has been calculated following organ extraction.
Results:
Organ specific differences of distribution were clearly demonstrated. Our results indicate that the kinetics of lymphoscintigraphic (99m)Tc-bound agents substantially depends on particle size. Reliable transport from the interstitium to initial lymph vessels and lymph node uptake suggested for tracers suited for lymphoscintigraphy a median size of about <100 nm.
Conclusion:
Our data could improve standardization of diagnostic methods and lead to an objective consideration of therapeutic procedures.
