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Updated: Aug 15, 2026
A Facile Protocol to Generate Site-Specifically Acetylated Proteins in Escherichia Coli
Published on: December 9, 2017
Selective deacetylation of zaluzanin D using transformed Escherichia coli cultures
Munuswamy Ramanujam Ganesh1, Jothi Arunachalam, Anand Krithika
1Department of Crystallography and Biophysics, University of Madras, Guindy Campus, Chennai 600 025, India.
Abstract:
The use of conventional and unconventional reaction methodology for the hydrolysis of the acetate group in zaluzanin D resulted in hydration of the 11,13 exocyclic bond along with deacetylation. But the microorganism E. coli selectively cleaved the acetate group to yield zaluzanin C.
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