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Updated: Sep 12, 2026

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Regulation of poly(ADP-ribose) polymerase gene expression in mitogen-stimulated human peripheral blood mononuclear
M Menegazzi1, A Carcereri de Prati, M Miwa
1Istituto di Chimica Biologica, Università di Verona, Italy.
Abstract:
The level of mRNA for poly(ADP-ribose) polymerase in human PBMC increased 8 h after addition of PHA, reaching its maximum (9-fold over the basal level) 3-4 days after the stimulation and decreasing thereafter. mRNA maximum slightly preceded in time the maximal value of DNA synthesis. The half-life of poly(ADP-ribose) polymerase mRNA, which is 1.2 h in quiescent PBMC, increased up to 3.4 h in stimulated PBMC. This PHA-induced stabilization of the mRNA for poly(ADP-ribose) polymerase could account for the accumulation of the transcript in mitogen-treated PBMC.
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