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Related Concept Videos

Protein-protein Interfaces02:04

Protein-protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Protein-Protein Interfaces02:04

Protein-Protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...

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Related Experiment Video

Updated: Jul 8, 2026

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells
08:38

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells

Published on: March 3, 2015

A human protein-protein interaction network: a resource for annotating the proteome.

Ulrich Stelzl1, Uwe Worm, Maciej Lalowski

  • 1Max Delbrueck Center for Molecular Medicine, 13092 Berlin-Buch, Germany.

Cell
|September 20, 2005
PubMed
Summary

This study systematically mapped human protein-protein interactions using yeast two-hybrid screening, revealing a large network of 3186 interactions. High-confidence interactions were identified, uncovering novel insights into cellular pathways and protein functions.

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Last Updated: Jul 8, 2026

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells
08:38

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells

Published on: March 3, 2015

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JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics
07:28

JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics

Published on: October 19, 2021

Area of Science:

  • Proteomics
  • Systems Biology
  • Molecular Biology

Background:

  • Protein-protein interactions (PPIs) are crucial for understanding cellular processes and proteome organization.
  • Systematic mapping of PPIs provides a comprehensive view of functional networks.

Purpose of the Study:

  • To systematically identify interacting pairs of human proteins.
  • To construct a large, highly connected human protein-protein interaction network.
  • To identify novel protein interactions and their roles in cellular pathways.

Main Methods:

  • Automated yeast two-hybrid (Y2H) interaction mating was employed to screen a matrix of 4456 baits and 5632 preys.
  • Independent pull-down and co-immunoprecipitation assays were used for validation.
  • Topological and Gene Ontology (GO) criteria were applied to score and define high-confidence interactions.

Main Results:

  • Identified 3186 mostly novel PPIs involving 1705 proteins, forming a large, highly connected network.
  • Validated the overall quality of Y2H interactions through independent assays.
  • Defined 911 high-confidence interactions among 401 proteins.
  • Discovered novel interactions linking uncharacterized proteins and disease proteins to regulatory pathways, including two validated Axin-1 interactions.

Conclusions:

  • Systematic PPI screens are effective for understanding protein function and cellular processes.
  • The generated human PPI network offers a valuable resource for further biological research.
  • Identified novel modulators of Wnt signaling, highlighting the utility of PPI mapping in disease-related pathway analysis.