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Heterogeneous nuclear ribonucleoprotein A2 is a SET-binding protein and a PP2A inhibitor

J Vera1, M Jaumot, J M Estanyol

  • 1Department of Cell Biology and Pathology, Faculty of Medicine, University of Barcelona, Barcelona, Spain.

Oncogene
|September 20, 2005
PubMed

Insights

The oncoprotein SET and hnRNPA2 protein cooperate to inhibit PP2A, stimulating cell proliferation. Overexpression of hnRNPA2 may drive oncogenesis by promoting cell growth.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Oncology

Background:

  • The oncoprotein SET regulates cell proliferation, likely by inhibiting cyclin B-cdk1 and protein phosphatase 2A (PP2A).
  • Heterogeneous nuclear ribonucleoprotein A2 (hnRNPA2) is involved in mRNA biogenesis.

Purpose of the Study:

  • To investigate the interaction between SET and hnRNPA2.
  • To elucidate the role of hnRNPA2 in PP2A inhibition and cell proliferation.

Main Methods:

  • In vitro and in vivo interaction studies between SET and hnRNPA2.
  • Analysis of hnRNPA2's effect on PP2A activity.
  • Cell proliferation assays upon hnRNPA2 overexpression.

Main Results:

  • SET interacts with hnRNPA2 via its RNP1 sequence within the RNA-binding domain (RBD).
  • hnRNPA2 inhibits PP2A activity, cooperating with SET, and requires its complete RBD for inhibition.
  • Overexpression of hnRNPA2, particularly its RBD, stimulates cell proliferation.

Conclusions:

  • hnRNPA2 acts as a PP2A inhibitor and promotes cell proliferation.
  • hnRNPA2's role in PP2A inhibition and cell proliferation suggests its potential involvement in oncogenesis, especially given its overexpression in tumors.

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