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RPC10 encodes a new mini subunit shared by yeast nuclear RNA polymerases
1DBCM, Section of Biochemistry and Molecular Genetics, Saclay Research Center, Gif-sur-Yvette, France.
Gene Expression
|January 1, 1992
Summary
The yeast ABC10 alpha subunit, encoded by RPC10, is essential for cell viability. This small protein plays a critical role in yeast RNA polymerases A, B, and C function.
Area of Science:
- Molecular Biology
- Yeast Genetics
Background:
- Yeast RNA polymerases A, B, and C share common small subunits.
- Two of these subunits, ABC10 alpha and ABC10 beta, co-migrate during SDS-PAGE analysis.
Purpose of the Study:
- To isolate and characterize the gene encoding the yeast ABC10 alpha subunit.
- To determine the functional significance of the ABC10 alpha subunit in yeast.
Main Methods:
- Gene isolation using microsequence data.
- Gene localization to chromosome VIII.
- Protein characterization, including identification of a zinc-binding domain.
- Gene deletion analysis to assess viability.
Main Results:
- The RPC10 gene was isolated and found to be a single-copy gene on chromosome VIII.
- RPC10 codes for a 70-amino acid basic protein with a CX2CX13CX2C zinc-binding motif.
- Deletion of the RPC10 gene resulted in yeast cell inviability, highlighting the subunit's essential nature.
- ABC10 alpha and ABC10 beta subunits exhibit limited sequence similarity.
Conclusions:
- The small ABC10 alpha subunit is indispensable for yeast cell viability.
- This subunit is a crucial component of yeast RNA polymerases.
- The zinc-binding domain may be important for ABC10 alpha function.