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Multidimensional modelling of anaerobic granules
C Picioreanu1, D J Batstone, M C M van Loosdrecht
1Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 Delft, The Netherlands. c.picioreanu@tnw.tudelft.nl
Summary
A new 3D biofilm model simulates microbial granules, accurately representing biochemical reactions and structure. This computational tool aids in interpreting observed microscopic and macroscopic features of biofilms.
Area of Science:
- Environmental microbiology
- Biochemical engineering
- Computational modeling
Background:
- Existing planar biofilm models lack the complexity to represent granule structure.
- Understanding microbial granule dynamics requires advanced simulation capabilities.
Purpose of the Study:
- To develop a multispecies, 3D biofilm model for simulating microbial granules.
- To incorporate biochemical reactions and individual-based biomass representation.
- To interpret microscopic and macroscopic granule features observed in situ.
Main Methods:
- Developed a 3D model based on a planar biofilm model and the ADM1 biochemical structure.
- Utilized an individual-based representation for biomass particles within granules.
- Employed an iterative pushing technique for biomass spreading and a Cartesian grid system.
Main Results:
- The model simulates substrate diffusion, reaction, and local pH changes within granules.
- It accurately represents both microscopic and macroscopic features of granule structure.
- The model avoids Cartesian artefacts common in grid-spreading techniques like cellular automata.
Conclusions:
- The developed 3D biofilm model provides a robust platform for studying microbial granules.
- It effectively integrates biochemical processes with spatial biomass dynamics.
- The model can be used to interpret in-situ observations of granule structure and function.