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Relationship between gelatinases and bone turnover in the healing bone defect
Richard A Reinhardt1, Hsi-ming Lee, Marian Schmid
1Departments of Surgical Specialties and Oral Biology, University of Nebraska Medical Center College of Dentistry, 40th and Holdrege Streets, Lincoln, NE 68583, USA. rareinha@unmc.edu
Summary
This study found that elevated gelatinase (MMP-9 and MMP-2) levels at two weeks after creating an alveolar bone defect correlated with decreased bone turnover and reduced bone fill, suggesting potential benefits of anti-MMP therapy.
Area of Science:
- Oral surgery and bone regeneration research.
- Biomarker analysis in wound healing.
Background:
- Soft tissue inflammation and bone turnover are critical in alveolar bone defect healing.
- Gelatinases (MMP-9, MMP-2) and bone turnover markers (OC, ICTP) are key indicators.
Purpose of the Study:
- To investigate the relationship between soft tissue inflammation markers (MMP-9, MMP-2) and bone turnover markers (OC, ICTP, bone fill) during alveolar bone defect healing.
- To assess the utility of a novel bone wash device for sampling at the bone/soft tissue interface.
Main Methods:
- A pilot study involving ten oral surgery subjects with a 5x5mm trephine defect.
- Bone wash sampling at baseline, 2 weeks, and 12 weeks post-surgery.
- Analysis of MMP-9, MMP-2, OC, ICTP, and albumin (ALB) using zymography, radioimmunoassays, and ELISA.
Main Results:
- All measured markers showed significant changes over time (P <= .03).
- Bone turnover markers (OC, ICTP) decreased, while gelatinases (MMP-9, MMP-2) increased at 2 weeks.
- Elevated MMP-9 and MMP-2 at 2 weeks were negatively correlated with ICTP and subsequent bone fill.
Conclusions:
- Gelatinase activity at 2 weeks post-defect creation may impede bone turnover and reduce bone fill.
- Findings suggest that anti-MMP therapy could be beneficial for enhancing wound healing in alveolar defects.