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Related Experiment Videos

Strontium-induced rat egg activation.

R Tomashov-Matar1, D Tchetchik, A Eldar

  • 1Cell and Developmental Biology and Physiology and Pharmacology, Sackler Faculty of Medicine, Tel Aviv University, Ramat-Aviv 9978 Tel-Aviv, Israel.

Reproduction (Cambridge, England)
|September 27, 2005
PubMed
Summary
This summary is machine-generated.

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Strontium chloride (SrCl2) effectively mimics sperm-induced rat egg activation by triggering calcium oscillations and cortical granule exocytosis. However, prolonged exposure may delay meiosis completion.

Area of Science:

  • Reproductive Biology
  • Developmental Biology
  • Cellular Physiology

Background:

  • Parthenogenetic agents inducing calcium oscillations mimic fertilization more closely than single calcium transients.
  • Strontium chloride (SrCl2) activates the inositol 1,4,5-trisphosphate receptor, evoking calcium oscillations.
  • Limited data exists on SrCl2-induced calcium oscillations and their effects on rat egg activation.

Purpose of the Study:

  • To investigate the effect of various SrCl2 concentrations on intracellular calcium ([Ca2+]i) oscillations in rat eggs.
  • To assess the impact of SrCl2-induced calcium oscillations on cortical granule exocytosis (CGE) and meiosis completion (CM).

Main Methods:

  • Rat eggs loaded with fura-2 were used to monitor [Ca2+]i oscillations.
  • Various concentrations of SrCl2 (2, 4, or 6 mM) were applied.

Related Experiment Videos

  • Cortical granule exocytosis (CGE) was assessed using immunohistochemistry and confocal microscopy.

    • Main Results:

    • 2 mM SrCl2 optimally induced an initial [Ca2+]i transient similar to sperm.
    • SrCl2-evoked oscillations had lower frequency and longer duration compared to sperm-activated eggs.
    • SrCl2 induced CGE comparable to sperm; however, 60-min exposure delayed meiosis completion (CM).

    Conclusions:

    • SrCl2 is an effective parthenogenetic agent for rat eggs, mimicking sperm-induced activation.
    • SrCl2 successfully triggers [Ca2+]i oscillations, CGE, and influences CM.
    • The pattern of [Ca2+]i oscillations differs between SrCl2 and sperm activation.