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Published on: January 17, 2014
[Study on the molecule epidemiological between resistances of 7 genes interrelated 4 antibiotic to isolated
Yun-fang Ding1, Zu-huang Mi, Jian-hua Zhang
1The Affiliated Children's Hospital of Suzhou University, Suzhou 215003, China.
Insights
Penicillin, erythromycin, and tetracycline resistance are high in pediatric Streptococcus pneumoniae isolates from Suzhou. Vancomycin remains sensitive, indicating a need for targeted antibiotic strategies.
Area of Science:
- Microbiology
- Genetics
- Epidemiology
Context:
- Streptococcus pneumoniae (SP) is a leading cause of childhood respiratory infections.
- Antibiotic resistance in SP poses a significant public health challenge.
- Understanding resistance mechanisms is crucial for effective treatment.
Purpose:
- To investigate the prevalence of antibiotic resistance genes in pediatric SP isolates.
- To determine the molecular basis of resistance to penicillin, erythromycin, tetracycline, and vancomycin.
- To analyze the genetic landscape of antibiotic resistance in SP from the Suzhou area.
Summary:
- Thirty-one pediatric SP isolates were analyzed for antibiotic susceptibility and resistance genes.
- High rates of resistance were observed for penicillin (61.3%), erythromycin (90.3%), and tetracycline (90.3%).
- Vancomycin susceptibility was 100%, with no vanA or vanB genes detected.
- Specific genes like pbp2B, ermA/B, mefA, and tetM were associated with observed resistance patterns.
Impact:
- Provides critical data on the antibiotic resistance profile of pediatric SP in Suzhou.
- Highlights the urgent need for antimicrobial stewardship and surveillance.
- Informs clinical decision-making for treating SP infections in children.
Objective:
To investigate the molecule epidemic for 7 genes interrelated penicillin, erythromycin, tetracycline, vancomycin resistance of isolated Streptococcus pneumoniae (SP) in children at Suzhou area.
Methods:
(1) Thirty-one pneumococcal isolates were collected from respiratory tract secretions of children with respiratory diseases from Nov 2002 to Apr 2003 at the Children's Hospital of Suzhou University (reference strain ATCC49619). (2) Penicillin susceptibility was determined by E-test, while erythromycin, tetracycline, vancomycin were determined by K-B disk. (3) The detecting of pbp2B, ermA/B, mefA, tetM, vanA, vanB genes by PCR, Sequencing pbp2B genes, Contrasting pbp2B DNA sequences among pneumococcal isolates and SP R6 [penicillin sensitive (www.ncbi.nlm.gov/nucleotide, NC-003098)].
Results:
Of thirty-one isolates studied, the results were shown as follows; (1) Penicillin sensibility 38.7% (n = 12), penicillin resistance 61.3% (n = 19), pbp2B mutation 64.5% (n = 20); (2) Erythromycin sensibility 9.7% (n = 3), erythromycin resistance 90.3% (n = 28), ermA/B 71% (n = 22), mefA 32.1% (n = 10), ermA/B + mefA 87.1% (n = 27); (3) Tetracycline sensibility 9.7% (n = 3), tetracycline resistance 90.3% (n = 28), tetM 90.3% (n = 28); (4) Vancomycin sensibility 100% (n = 31), vanA, vanB all 0%.
Conclusion:
Among pneumococcal isolates at our area, penicillin, erythromycin, tetracycline resistance were high, vancomycin was sensitive. Detecting 7 genes interrelated penicillin, erythromycin, tetracycline, vancomycin resistance expressed genotypies for antibiotic resistances in pneumococcal isolates.
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