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Related Experiment Videos

DING proteins are from Pseudomonas.

Alan P Lewis1, Daniel Crowther

  • 1Bioinformatics Discovery and Analysis, GlaxoSmithKline Medicines Research Centre, Stevenage, UK. alan.p.lewis@gsk.com

FEMS Microbiology Letters
|September 28, 2005
PubMed
Summary
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DING proteins, previously thought to exist in plants and animals, may not be eukaryotic. Analysis suggests these sequences likely originate from microbial contamination, not true animal or plant proteins.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Genomics

Background:

  • DING proteins were characterized by an N-terminal DINGGG-sequence and proposed roles in biomineralization, signaling, or as receptors in eukaryotes.
  • Previously, DING sequences were only found as N-terminal peptides or partial cDNA, not in available animal and plant genomes.
  • Microbial homologs functioning as periplasmic phosphate-binding proteins have been identified.

Purpose of the Study:

  • To investigate the true origin and existence of DING proteins in eukaryotes.
  • To re-evaluate the classification of DING protein sequences found in public databases.

Main Methods:

  • Sequencing of full-length genes from *Pseudomonas aeruginosa* and *Hypericum perforatum*.
  • Database searches using newly sequenced genes to identify homologous plant and animal cDNA fragments.

Related Experiment Videos

  • Analysis of codon usage in identified DNA sequences.

    • Main Results:

    • Full-length genes from *P. aeruginosa* and *H. perforatum* showed homology to known DING protein sequences.
    • Searches identified additional plant and animal cDNA fragments and a rat genomic contig with DING-like sequences.
    • Codon usage analysis indicated all identified sequences were of *Pseudomonas* origin.

    Conclusions:

    • DING proteins likely do not exist in eukaryotes.
    • Observed DING sequences in plant and animal databases are potentially artifacts of microbial contamination or infection.