Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Function of a novel brain-specific gene LRRC4].

Song-qing Fan1, Jie-ru Wang, He Huang

  • 1Cancer Research Institute, Central South University, Changsha 410078, China.

Zhonghua Zhong Liu Za Zhi [Chinese Journal of Oncology]
|September 29, 2005
PubMed
Summary

The LRRC4 gene suppresses human glioma U251 cell growth by inhibiting DNA synthesis and proliferation. This gene restrains cell cycle progression, indicating its role in tumor suppression.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Isomeric Dibenzodiindenophenanthrene Diradicaloids: Synthesis, Structures, Properties, and Efficient NIR Photothermal Conversion for Imaging.

Angewandte Chemie (International ed. in English)·2026
Same author

Early onset primary angle closure and foveal retinoschisis associated with a pseudo-homozygous CRB1 pathogenic variant.

Documenta ophthalmologica. Advances in ophthalmology·2026
Same author

Long-term outcomes of one-stop unilateral thoracoscopic hybrid ablation for persistent atrial fibrillation after failed catheter ablation.

Journal of cardiothoracic surgery·2026
Same author

The KIF6-RBP Complex Orchestrates mRNA Transport Required for Sperm Flagellar Assembly.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Psychosocial pathways linking type D personality traits to quality of life in patients with permanent intestinal stomas: a cross-sectional structural equation modeling study.

Frontiers in medicine·2026
Same author

A Two-Stage Deep Learning Method for Non-Invasive Sow Body Temperature Prediction Fusing Thermal Imaging and Environmental Parameters.

Animals : an open access journal from MDPI·2026

Area of Science:

  • Molecular Biology
  • Genetics
  • Oncology

Context:

  • Glioblastoma is an aggressive brain tumor with limited treatment options.
  • Understanding the genetic factors influencing glioma progression is crucial for developing new therapies.

Purpose:

  • To investigate the tumor-suppressive effects of the LRRC4 gene in human glioma U251 cells.
  • To elucidate the biological functions of LRRC4 in glioma.

Summary:

  • LRRC4 gene transfection in U251 cells led to decreased cell morphometric parameters, DNA content, and AgNORs.
  • LRRC4 induced G(0)/G(1) cell cycle arrest and reduced proliferation, as evidenced by flow cytometry and MTT assays.
  • These findings suggest LRRC4 acts as a tumor suppressor by inhibiting DNA synthesis and cell proliferation.

Related Experiment Videos

Impact:

  • LRRC4 gene may serve as a potential therapeutic target for glioma treatment.
  • The study highlights the utility of combining morphometry with flow cytometry and MTT assays for gene function analysis.