Related Experiment Video
Updated: Aug 15, 2026
10:07
In Vivo Two-photon Imaging Of Experience-dependent Molecular Changes In Cortical Neurons
Published on: January 5, 2013
In vivo brain imaging using a portable 3.9 gram two-photon fluorescence microendoscope
Benjamin A Flusberg1, Juergen C Jung, Eric D Cocker
1James H. Clark Center for Biomedical Engineering & Sciences, Stanford University, Stanford, California 94305-5435, USA.
Optics Letters
|September 30, 2005
Summary
We developed a compact, portable two-photon fluorescence microendoscope for in vivo brain imaging. This advanced microendoscope achieved micrometer-scale resolution, enabling visualization of hippocampal blood vessels in live mice.
Area of Science:
- Neuroscience
- Biomedical Engineering
- Optical Imaging
Background:
- Advanced imaging techniques are crucial for understanding brain function and disease.
- Existing microendoscopes often face limitations in size, resolution, or portability.
Purpose of the Study:
- To develop a compact and portable two-photon fluorescence microendoscope system.
- To demonstrate its capability for in vivo neurovascular imaging.
Main Methods:
- Utilized a compound gradient refractive index endoscope probe.
- Incorporated a DC micromotor for remote image plane adjustment.
- Employed a flexible photonic bandgap fiber for ultrashort pulse delivery.
- Developed a lightweight imaging head (3.9 g).
Main Results:
- Achieved micrometer-scale resolution.
- Demonstrated near distortion-free delivery of ultrashort excitation pulses.
- Successfully visualized hippocampal blood vessels in live mice using portable two-photon microendoscopy.
Conclusions:
- The developed microendoscope is a compact, high-resolution tool for in vivo neuroscience research.
- It offers a portable solution for advanced brain imaging applications.
- This technology facilitates detailed study of neurovascular dynamics in animal models.
