Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Volatile anesthetics bind rat synaptic snare proteins.

Peter Nagele1, J Brett Mendel, William J Placzek

  • 1Department of Anesthesiology, Washington University School of Medicine, St. Louis, MO 63110, USA.

Anesthesiology
|September 30, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Raptor and Drp1 Function Synthetically to Control Hypoxic Death and the Mitochondrial Network in Caenorhabditis elegans.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026
Same author

Myeloid Cell Leukemia 1 and Hexokinase 2 Directly Interact to Form a Glucose Metabolic Regulatory Axis.

Cells·2026
Same author

Suicidal behaviour after surgery.

British journal of anaesthesia·2026
Same author

Fully automated, deep learning, cardiac CT-based multimodal network for cardiovascular risk stratification in high-risk perioperative patients.

European heart journal. Digital health·2026
Same author

Rapid antidepressant potential of nitrous oxide: current state and major questions.

Molecular psychiatry·2025
Same author

Transpulmonary variation in cardiac biomarker concentrations: a pilot study in cardiac surgery patients.

British journal of anaesthesia·2025

Volatile anesthetics bind to SNARE proteins, including syntaxin and SNAP-25B, suggesting these proteins are key synaptic targets. This research identifies potential binding sites for anesthetics at the synapse.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Pharmacology

Background:

  • Volatile anesthetics (VAs) inhibit excitatory neurotransmitter release, but their presynaptic targets remain unidentified.
  • Genetic studies in C. elegans suggest a protein interacting with syntaxin 1A is a VA target.
  • This study investigates the binding of VAs to syntaxin 1A and associated proteins.

Purpose of the Study:

  • To identify presynaptic binding proteins for volatile anesthetics.
  • To test the hypothesis that proteins interacting with syntaxin 1A are VA targets.
  • To characterize the binding of halothane and isoflurane to SNARE proteins.

Main Methods:

  • Recombinant rat syntaxin 1A, SNAP-25B, and VAMP2 were tested for VA binding.
  • F-nuclear magnetic resonance (NMR) relaxation measurements were used to detect binding.

Related Experiment Videos

  • Circular dichroism examined structural alterations and complex formation.

    • Main Results:

    • VAs did not bind to VAMP2.
    • VAs bound to SNAP-25B homomultimers and to syntaxin 1A and the SNARE complex at clinical concentrations.
    • A His6 tag on syntaxin abolished VA binding, indicating specific structural requirements for the binding site.

    Conclusions:

    • SNARE proteins, including syntaxin and SNAP-25B, are identified as potential synaptic targets for volatile anesthetics.
    • The binding of VAs to SNARE proteins suggests a mechanism for anesthetic-induced inhibition of neurotransmitter release.