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Published on: October 29, 2020
[Interaction between balofloxacin and DNA and the influence of Mg2+ on the interaction]
Wen-Ying Zhong1, Qi Huang, Dan-Dan Wang
1Department of Analytical Chemistry, China Pharmaceutical University, Nanjing 210038, China. wenyingzhongnj@163.com
Aim:
To study the binding mode of balofloxacin with DNA and evaluate the influence of Mg2+ on the binding between balofloxacin and DNA.
Methods:
Fluorescent spectroscopy was used to study the interaction of balofloxacin with DNA and to calculate the thermodynamic constants. UV-Vis spectra, DNA viscosity titration, competition experiment and the effect of dsDNA and ssDNA on the fluorescense intensity were used to identify the binding mode.
Results:
Balofloxacin interacted with CT-DNA with a quenching constant of (5.43 +/- 0.07) x 10(3) L x mol(-1). The interaction was exothermic with a Van't Hoff enthalply of - 8.03 kJ x mol(-1) x Mg2+ cation could enhance the quenching constant between balofloxacin and DNA.
Conclusion:
Balofloxacin interacted with CT-DNA in the mode of groove binding and Mg2+ could mediate the binding of balofloxacin to DNA.
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