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Related Experiment Videos

Monitoring a mouse colony for Helicobacter bilis using a Helicobacter-genus-specific nested PCR.

K Jacobsen1, E Mahabir, M Brielmeier

  • 1Department of Comparative Medicine, GSF-National Research Centre for Environment and Health, Neuherberg, Germany.

Laboratory Animals
|October 4, 2005
PubMed
Summary

A new nested PCR method offers a highly sensitive way to detect Helicobacter bilis infections in mice. Analyzing fecal pellets is advantageous, allowing early detection without harming the animals and providing colony-wide health insights.

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Area of Science:

  • Veterinary Microbiology
  • Laboratory Animal Science
  • Molecular Diagnostics

Background:

  • Subclinical Helicobacter infections in laboratory mice can compromise experimental data integrity.
  • Accurate identification of murine Helicobacter species is crucial for reliable research outcomes.

Purpose of the Study:

  • To develop a highly sensitive diagnostic method for detecting Helicobacter infections in mice.
  • To evaluate the efficacy of different sample types and timing for H. bilis detection.

Main Methods:

  • Development and validation of a nested polymerase chain reaction (PCR) assay.
  • Experimental infection of C3H/HeJ and C57BL/6 mice with H. bilis.
  • Analysis of various tissues (faeces, caecum, colon, rectum, liver) and fecal pellets for H. bilis DNA.

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Main Results:

  • Nested PCR demonstrated 10-100 times greater sensitivity than single-step PCR.
  • Faeces, caecum, colon, and rectum were suitable for H. bilis detection; liver was not.
  • H. bilis was detectable in faecal pellets one week post-infection, avoiding animal euthanasia.
  • Fecal analysis provided representative data for cagemates and allowed repeated testing.
  • Breeding pair fecal samples accurately reflected colony Helicobacter status.
  • Both mouse strains were susceptible to persistent H. bilis infection over 20 weeks.

Conclusions:

  • Nested PCR of pooled fecal pellets is the most sensitive method for monitoring H. bilis in mouse colonies.
  • Fecal analysis offers a non-invasive, repeatable, and highly sensitive approach for murine Helicobacter diagnostics.