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Related Experiment Videos

Eomesodermin is a localized maternal determinant required for endoderm induction in zebrafish.

Christopher R R Bjornson1, Kevin J P Griffin, Gist H Farr

  • 1Department of Biochemistry, University of Washington, Seattle, Washington 98195-7350, USA.

Developmental Cell
|October 4, 2005
PubMed
Summary

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Eomesodermin (Eomes) is crucial for zebrafish endoderm development. This T-box transcription factor works with Gata5 and Bon to activate the Casanova gene, initiating endoderm formation.

Area of Science:

  • Developmental biology
  • Molecular genetics
  • Zebrafish embryogenesis

Background:

  • Endoderm induction in zebrafish is essential for organogenesis.
  • Casanova (Cas) is the earliest known endoderm-specific transcription factor.
  • Gata5 and Bon are necessary for Cas expression but cannot induce it in all cells.

Purpose of the Study:

  • To identify the missing factor(s) required for Cas expression and endoderm induction.
  • To elucidate the molecular mechanism by which Cas expression is regulated.
  • To determine the role of Eomesodermin (Eomes) in early zebrafish development.

Main Methods:

  • Expression analysis of Casanova (Cas) in zebrafish embryos.
  • Functional assays involving transcription factors Gata5, Bon, and Eomesodermin (Eomes).

Related Experiment Videos

  • Analysis of Eomes binding to the Cas promoter and protein-protein interactions.

    • Main Results:

    • Eomesodermin (Eomes), a maternally localized T-box transcription factor, is essential for Cas expression.
    • Eomes synergizes with Gata5 and Bon to induce Cas even in animal pole blastomeres.
    • Eomes binds to a critical site in the Cas promoter and physically interacts with Gata5 and Bon.

    Conclusions:

    • Eomesodermin (Eomes) is a key regulator of endoderm induction in zebrafish.
    • Eomes, Gata5, and Bon form a transcriptional complex to activate Cas expression.
    • This complex facilitates endoderm formation by assembling on the Cas promoter.