Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Computer modeling of gibberellin-DNA binding.

F H Witham1, L B Hendry

  • 1Department of Horticulture, Pennsylvania State University, University Park 16802.

Journal of Theoretical Biology
|March 7, 1992
PubMed
Summary

Gibberellins can intercalate into DNA dinucleotides, stabilizing the DNA structure through molecular interactions. Further research is needed to determine if this mechanism affects DNA uncoiling or gene expression.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Adolescents and television.

Journal of youth and adolescence·2014
Same author

Body type, body esteem, school, and leisure: A study of overweight, average, and underweight adolescents.

Journal of youth and adolescence·2014
Same author

Attractive and inhibitory pheromones produced in the bark beetle,Dendroctonus brevicomis, during host colonization: Regulation of inter- and intraspecific competition.

Journal of chemical ecology·2013
Same author

The influence of N-dialkyl and other cationic substituents on DNA intercalation and genotoxicity.

Mutation research·2005
Same author

Young people in 'drinking' societies? Norwegian, Scottish and Swedish adolescents' perceptions of alcohol use.

Health education research·2001
Same author

Novel piperidinedione analogs as inhibitors of breast cancer cell growth.

Archiv der Pharmazie·2001

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Computational Chemistry

Background:

  • Gibberellins are plant hormones with diverse biological functions.
  • DNA intercalation is a known mechanism for drug-DNA interactions.
  • The interaction between gibberellins and DNA has not been extensively studied.

Purpose of the Study:

  • To investigate the potential of gibberellins to intercalate into DNA.
  • To explore the molecular interactions governing gibberellin-DNA complex formation.
  • To assess the implications of gibberellin intercalation for DNA structure and function.

Main Methods:

  • Computer modeling and molecular mechanics simulations were employed.
  • Analysis of intercalation complexes between gibberellins and DNA dinucleotides (5'-dTdA-3').
  • Evaluation of energetic and entropic contributions to complex stabilization.

Main Results:

  • Gibberellins were shown to intercalate between base pairs of the 5'-dTdA-3' dinucleotide.
  • Complex formation is stabilized by hydrogen bonding and van der Waals interactions.
  • Optimal binding geometry and stereochemical recognition were observed, dependent on component chirality.

Conclusions:

  • Gibberellins can form stable intercalation complexes with DNA dinucleotides.
  • The stereochemistry of gibberellins and DNA dinucleotides is crucial for effective binding.
  • The potential role of gibberellins in DNA uncoiling and gene regulation warrants further investigation.

Related Experiment Videos