Related Experiment Video
Updated: Aug 15, 2026

Preparation of Single-Cell Suspension of Mouse Thymic Epithelial Cells and Staining of Intracellular Molecules for Flow Cytometric Analysis
Published on: July 26, 2024
Central tolerance: learning self-control in the thymus
Kristin A Hogquist1, Troy A Baldwin, Stephen C Jameson
1Center for Immunology, Department of Laboratory Medicine and Pathology, University of Minnesota, 312 Church Street South East, Minneapolis, Minnesota 55455, USA. hogqu001@umn.edu
Abstract:
In the past few years, there has been a flurry of discoveries and advancements in our understanding of how the thymus prepares T cells to exist at peace in normal healthy tissue: that is, to be self-tolerant. In the thymus, one of the main mechanisms of T-cell central tolerance is clonal deletion, although the selection of regulatory T cells is also important and is gaining enormous interest. In this Review, we discuss the emerging consensus about which models of clonal deletion are most physiological, and we review recent data that define the molecular mechanisms of central tolerance.
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