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Updated: Aug 15, 2026

Production of Replication-Defective Retrovirus by Transient Transfection of 293T cells
Published on: December 4, 2007
[Study on the expression of human acute myeloid leukemia retrovirus gene]
1Department of Hematology, The Second Affiliated Hospital, Medical College of Zhejiang University, Hangzhou, China.
Objective:
To explore the potentiality of retroviral etiology in human acute myeloid leukemia (AML).
Methods:
The expression of retrovirus clone 6 # 11 in leukemic cells from 19 AML patients and peripheral blood mononuclear cells (PBMNCs) from 20 controls was studied by Northern blot and reverse transcriptase polymerase chain reaction (RT-PCR).
Results:
Northern blot revealed the positive bands of 9.4 kb and 4.5 kb in 18 of 19 AML patients, and RT-PCR a 790 bp product in 11 of 14 AML patients.
Conclusion:
There was a specific expression of acute myeloid leukemia associated retrovirus in AML patients.
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