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Novel BOD optical fiber biosensor based on co-immobilized microorganisms in ormosils matrix
Ling Lin1, Lai-Long Xiao, Sha Huang
1Department of Chemistry and Key Laboratory of Analytical Sciences of the Ministry of Education, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
Biosensors & Bioelectronics
|October 6, 2005
Summary
A novel biochemical oxygen demand (BOD) sensor utilizes an optical fiber and immobilized microorganisms for accurate water quality assessment. This innovative sensor provides reliable BOD measurements in seawater samples, correlating well with traditional methods.
Area of Science:
- Environmental Science
- Biotechnology
- Analytical Chemistry
Background:
- Biochemical oxygen demand (BOD) is a critical indicator of water quality.
- Conventional BOD testing methods are time-consuming and labor-intensive.
- Development of rapid and reliable BOD sensors is essential for effective environmental monitoring.
Purpose of the Study:
- To develop and characterize a novel optical sensor for rapid biochemical oxygen demand (BOD) measurement.
- To evaluate the performance of the BOD sensor in seawater samples.
- To investigate the influence of environmental factors on sensor stability and accuracy.
Main Methods:
- Fabrication of a sensing film using organically-modified silicate (ORMOSIL) embedded with a ruthenium complex and immobilized seawater microorganisms.
- Utilizing a dissolved oxygen (DO) optical fiber within a light-proof cell for kinetic mode measurements.
- Testing the sensor's response using glucose/glutamate (GGA) standard solutions and real seawater samples.
Main Results:
- The developed optical BOD sensor demonstrated a determination range of 0.2 to 40 mg/L GGA.
- Sensor performance was evaluated under varying temperature, pH, and salinity conditions.
- BOD values obtained from the optical sensor showed good correlation with the conventional BOD5 method for seawater samples.
Conclusions:
- The immobilized microorganism-based optical BOD sensor offers a promising alternative for efficient water quality monitoring.
- The sensor exhibits good stability and accuracy, making it suitable for analyzing seawater samples.
- This technology has the potential to significantly reduce the time and resources required for BOD analysis.