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Updated: Aug 15, 2026

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells
Published on: March 16, 2017
Ascorbic acid enhances nitric oxide production in trabecular meshwork cells
1Department of Ophthalmology, Catholic University of Daegu College of Medicine, Daegu, Korea. jwkim@cu.ac.kr
Purpose:
This study investigated the role of ascorbic acid on the production of nitric oxide (NO) in the trabecular meshwork (TM) cells.
Methods:
After primarily cultured human TM cells were exposed to 1, 10, and 100 microM of L-ascorbic acid (LAA), with or without co-administration of 1 mM sodium nitroprusside or 100 microM hydrogen peroxide for 48 hr, cellular survival and NO production were measured with MTT and Griess assay, respectively.
Results:
LAA significantly potentiated NO production in a dose-dependent manner (p<0.05) without affecting cell viability. LAA increased cell viability after hydrogen peroxide-induced oxidative stress in a dose-dependent manner. LAA enhanced NO production in TM cells and showed a cytoprotective effect against hydrogen peroxide-induced oxidative stress.
Conclusions:
LAA might be involved in the regulation of trabecular outflow by enhancing NO production in TM cells.
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