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Updated: Aug 15, 2026
A Translational Surgical Porcine Model for Postoperative Intra-Abdominal Adhesion Formation
Published on: March 13, 2026
Micronized purified flavonoid fraction may prevent formation of intraperitoneal adhesions in rats
Hatice Gulsen Yilmaz1, Ibrahim Halil Tacyildiz, Celalettin Keles
1Department of General Surgery, Faculty of Medicine, University of Dicle, Diyarbakir, Turkey. gyilmaz@dicle.edu.tr
Objective:
To evaluate the efficacy of an anti-inflammatory and capillary regulator drug, micronized purified flavonoid fraction (MPFF), in the prevention of postoperative formation of adhesions.
Design:
A double-blind, controlled study evaluated the efficacy of MPFF in reducing postoperative adhesion formation in a rat model.
Setting:
Animal care facility of an academic research setting.
Animal(S):
Thirty Sprague-Dawley female rats randomly divided into three groups.
Intervention(S):
Starting on day of surgery, group 2 rats received oral MPFF (100 mg/kg per day for 7 days). Group 3 rats were intraperitoneally injected with 5 mL of saline (containing 200 mg/kg per day of MPFF for 3 days). Control rats received no medication. A standardized surgical trauma was applied in all animals. Three weeks after surgery, the rats were killed, and the adhesions were scored according to macroscopic and microscopic scales.
Main Outcome Measure(S):
Postoperative adhesions.
Result(S):
Both oral and intraperitoneal administration of MPFF reduced the scores of adhesions according to macroscopic and microscopic scales. There was no difference between the routes of administration.
Conclusion(S):
A statistically significant reduction in postoperative formation of adhesions was observed after oral and intraperitoneal administration of MPFF in our experimental animal model. However, further studies are required to reveal its mechanism of action.
Insights
Micronized purified flavonoid fraction (MPFF) effectively reduced postoperative adhesions in a rat model via oral or intraperitoneal administration. Further research is needed to understand its mechanism of action.
Area of Science:
- Surgical research
- Pharmacology
- Inflammation and immunology
Background:
- Postoperative adhesions are a significant complication following abdominal surgery.
- Adhesions can lead to chronic pain, bowel obstruction, and infertility.
- Current prevention strategies have limitations.
Purpose of the Study:
- To evaluate the efficacy of micronized purified flavonoid fraction (MPFF) in preventing postoperative adhesion formation.
- To compare the effectiveness of oral versus intraperitoneal administration of MPFF.
Main Methods:
- A randomized, double-blind, controlled study was conducted in a rat model.
- Thirty Sprague-Dawley female rats were divided into three groups: control, oral MPFF, and intraperitoneal MPFF.
- MPFF was administered starting on the day of surgery, with adhesion scoring performed three weeks post-surgery.
Main Results:
- Both oral and intraperitoneal administration of MPFF significantly reduced the incidence and severity of adhesions.
- Macroscopic and microscopic adhesion scores were lower in MPFF-treated groups compared to controls.
- No significant difference was observed between the oral and intraperitoneal routes of MPFF administration.
Conclusions:
- MPFF demonstrates significant efficacy in reducing postoperative adhesion formation in a preclinical animal model.
- Both oral and intraperitoneal administration routes are effective.
- Further investigation is warranted to elucidate the precise mechanism of action of MPFF in adhesion prevention.
