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Updated: Aug 15, 2026
Orthotopic Ovarian Transplantation Procedures to Investigate the Life- and Health-span Influence of Ovarian Senescence in Female Mice
Published on: February 12, 2018
Reproductive aging results in a reconfigured ovarian antioxidant defense profile in rats
John Yeh1, Mary Joann Bowman, Richard W Browne
1Department of Gynecology and Obstetrics, University at Buffalo, Buffalo, New York 14222, USA. jyeh@buffalo.edu
Objective:
To test our hypothesis that reproductive aging changes the ovarian oxidative stress defense profile, in response to prostaglandin F2alpha (PGF2alpha) during corpus luteum regression, because how a cell or an organ handles reactive oxygen intermediates may be dependent on the biological age of the organism.
Design:
Animal experimentation using rat model of corpus luteum regression.
Setting:
University reproductive biology laboratory.
Animal(S):
Control (26-day-old) and 8- to 9-month-old (reproductive aging) rats.
Intervention(S):
Corpus luteum formation was induced in control and 8- to 9-month-old (reproductive aging) rats with pregnant mare serum gonadotropin followed by human chorionic gonadotropin. Regression was then initiated with PGF2alpha.
Main Outcome Measure(S):
Vitamin E, glutathione reductase, glutathione peroxidase, catalase, and thiobarbituric acid-reacting substances were measured.
Result(S):
Ovaries from reproductive aging rats, compared with the control (26-day-old) group, had elevated vitamin E levels at 0, 2, and 24 hours after PGF2alpha. At 2 and 24 hours after PGF2alpha, the aging ovaries had lower glutathione reductase levels.
Conclusion(S):
These data suggest that the reproductive aging ovary has a transformed oxidative stress defense profile and that this may account for some of the physiological changes found in reproductive aging.
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