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Updated: Aug 15, 2026

Ovarian Tissue Culture to Visualize Phenomena in Mouse Ovary
Published on: June 19, 2018
Expression of plexin-B1 in the mouse ovary and its possible role in follicular development
Avital Regev1, Shlomit Goldman, Eliezer Shalev
1Department of Obstetrics and Gynecology, Ha'Emek Medical Center, Afula, Israel.
Objective:
To study the expression of plexin-B1 in the mouse ovary and its possible role in follicular development.
Design:
Controlled laboratory study.
Setting:
Research laboratory, Ha'Emek Medical Center.
Patient(S):
ICR female mice (n = 36) were studied.
Main Outcome Measure(S):
Plexin-B1 expression by immunohistochemistry, Western blot analysis, and reverse transcriptase-polymerase chain reaction. Microscopic morphometric and morphologic analysis for the evaluation of mouse follicular growth and E2, P, and T secretion from the developed follicles.
Result(S):
Plexin-B1 was expressed in the mouse ovary mostly in the granulosa cells. Plexin-B1 gene expression increased significantly by 126.8% +/- 38% in the pregnant mare serum gonadotropin-treated group and increased by 98.7% +/- 15.2 and 262.2% +/- 65.4% in the hCG-treated group 6 h and 24 h after injection, respectively (ANOVA; P < .05). Comparison of the maximum diameters of the control and study groups on day 9 of the culture period revealed a significant difference between the two groups (P < .001). In the control group, the mean maximum diameter was 191 +/- 2.6 microm compared with 136 +/- 5.13 microm in the presence of neutralizing antibodies against plexin-B1. Neutralizing antibody against plexin-B1 decreases E2 and P secretion.
Conclusion(S):
This study shows for the first time the expression of plexin-B1 in the mouse ovary. A possible role for plexin-B1 in follicular growth and steroidogenesis, most likely via the granulosa cells, is suggested.
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