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Related Experiment Video

Updated: Jan 10, 2026

Simulation of a Scaled Assembly Process with Collaboration of a Robotic Arm and Monitoring through a Vision System for Quality Control

Simulation of a Scaled Assembly Process with Collaboration of a Robotic Arm and Monitoring through a Vision System for Quality Control

Published on: August 29, 2025

Methods and results for semi-automated cloning using integrated robotics.

Heath E Klock¹, Aprilfawn White, Eric Koesema

¹Joint Center for Structural Genomics, Genomics Institute of the Novartis Research Foundation, 10675 John Jay Hopkins Drive, San Diego, CA 92121, USA.

Journal of Structural and Functional Genomics

|October 8, 2005

Summary

The Joint Center for Structural Genomics developed automated cloning methods to increase efficiency. This robotic platform enables high-throughput cloning, requiring less manpower and reducing costs.

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Area of Science:

Structural biology
Genomics
Biotechnology

Background:

The Joint Center for Structural Genomics (JCSG) prioritizes automation and parallel processing.
High-throughput cloning is essential for structural genomics initiatives.

Purpose of the Study:

To describe automated methods for high-throughput cloning.
To present results from JCSG projects demonstrating the utility of automated cloning.

Main Methods:

Automation of Polymerase Chain Reaction (PCR), restriction digests, ligations, gel electrophoresis, and microtiter plate assays.
Implementation of a robotic platform for parallel processing of molecular cloning steps.

Main Results:

The automated system can process 384 clones weekly, supplying expression and purification pipelines.
94% of generated PCR amplicons were successfully cloned and verified by sequencing (83% of total targets).

Conclusions:

The robotic platform facilitates rapid, cost-effective, and low-manpower high-throughput cloning.
The developed methods provide insights for optimizing cloning protocols in structural genomics.