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Changes in growth factor levels in human wound fluid
V M Dvonch1, R J Murphey, J Matsuoka
1Department of Ophthalmology, College of Medicine, University of South Florida, Tampa.
Surgery
|July 1, 1992
Summary
Platelet-derived growth factor (PDGF) and monocyte/macrophage-derived growth factor (MDGF) levels change over time after surgery, supporting wound repair models. MDGF may be crucial for healing, acting on cells unresponsive to PDGF AA.
Area of Science:
- Wound healing research
- Cell signaling in tissue repair
Background:
- Platelet-derived growth factor (PDGF) is implicated in wound healing.
- Human wound fluid contains PDGF AA and monocyte/macrophage-derived growth factor (MDGF) post-surgery.
Purpose of the Study:
- To analyze the temporal changes in PDGF and MDGF levels and activity in human wound fluid.
- To investigate the specific role of MDGF in wound healing.
Main Methods:
- Assayed PDGF AA concentration using Western blot.
- Determined chemotactic and mitogenic potential of wound fluid components.
- Assayed MDGF biologic activity on cell lines unresponsive to PDGF AA.
Main Results:
- PDGF AA and MDGF concentrations and biologic activity peaked immediately post-surgery, declining within 24 hours.
- MDGF chemotactic activity also peaked early and decreased over 24 hours.
- MDGF demonstrated biologic activity on cell lines unresponsive to PDGF AA.
Conclusions:
- Observed changes in PDGF and MDGF levels support a cascade model of wound repair.
- MDGF's distinct activity suggests a significant, potentially independent, role in wound healing.