Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The platelet microparticle proteome.

Benjamin A Garcia1, David M Smalley, Hyungjun Cho

  • 1Department of Chemistry, University of Virginia, Charlottesville, VA 22904, USA.

Journal of Proteome Research
|October 11, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The conserved N-terminal SANT1-binding domain (SBD) of EZH2 regulates PRC2 activity.

Genes & development·2026
Same author

Editorial Expression of Concern: Direct targeting of Sec23a by miR-200s influences cancer cell secretome and promotes metastatic colonization.

Nature medicine·2026
Same author

Defining the heterogeneous molecular landscape of lung cancer cell responses to epigenetic inhibition.

Communications biology·2026
Same author

Nuclear N-Glycosylation Redefines the Glycoscape and Directs Cell Identity.

bioRxiv : the preprint server for biology·2025
Same author

Pseudouridine selects RNAs for extracellular transport.

bioRxiv : the preprint server for biology·2025
Same author

DNASE1L3 surveils mitochondrial DNA on the surface of distinct mammalian cells.

bioRxiv : the preprint server for biology·2025
Same journal

Molecular Solution to the Paradox of Ancient Brain Preservation.

Journal of proteome research·2026
Same journal

From Method-Defined Signals to Reference Measurement Procedures: Two Decades of Mass Spectrometry-Based ProGRP Quantification.

Journal of proteome research·2026
Same journal

Proteomic Profiling of Extracellular Vesicle-Enriched Plasma Using Mag-Net for Biomarker Discovery in Pancreatic Ductal Adenocarcinoma.

Journal of proteome research·2026
Same journal

Computationally Efficient Bayesian Estimation of Graphical Networks for Omics Data.

Journal of proteome research·2026
Same journal

Hierarchy of MS-Based Evidence.

Journal of proteome research·2026
Same journal

Proteomic Profiling of Exosomes from HPV-Positive and HPV-Negative Head and Neck Squamous Cell Carcinoma: Selective Cargo Packaging.

Journal of proteome research·2026
See all related articles

This study presents the first proteomic analysis of activated platelet microparticles, identifying 578 proteins. These findings advance understanding of microparticle roles in health and disease.

Area of Science:

  • Proteomics
  • Hematology
  • Cell Biology

Background:

  • Platelet-derived microparticles are abundant in blood.
  • They play significant biological roles.
  • Their comprehensive proteomic profile remains largely uncharacterized.

Purpose of the Study:

  • To perform the first proteomic analysis of microparticles derived from activated human platelets.
  • To identify proteins within these microparticles.
  • To compare findings with existing platelet proteome studies.

Main Methods:

  • Proteomic analysis using 1D SDS-PAGE.
  • Liquid chromatography coupled to linear ion trap mass spectrometry (LC-MS/MS).
  • Protein identification based on MS/MS detections and unique peptides.

Related Experiment Videos

Main Results:

  • Identification of 578 unique proteins in activated platelet microparticles.
  • Detection of known platelet proteins like GPIIIa, GPIIb, P-selectin, CXCL4, CXCL7, and CCL5.
  • Discovery of approximately 380 novel proteins not previously reported in platelet proteome studies.

Conclusions:

  • This proteomic dataset provides a comprehensive view of activated platelet microparticle composition.
  • The identified proteins offer new insights into microparticle formation and function.
  • Findings may facilitate future research into the role of microparticles in various disease states.