Related Experiment Video
Updated: Aug 15, 2026

Syntheses, Crystallization, and Spectroscopic Characterization of 3,5-Lutidine N-Oxide Dehydrate
Published on: April 24, 2018
2-hydrazino-1-methylpyridine: a highly sensitive derivatization reagent for oxosteroids in liquid
Tatsuya Higashi1, Akinori Yamauchi, Kazutake Shimada
1Division of Pharmaceutical Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan. higashi@p.kanazawa-u.ac.jp
Abstract:
A derivatization reagent, 2-hydrazino-1-methylpyridine, was developed for the liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) of oxosteroids. The reagent quantitatively reacted with oxosteroids at 60 degrees C within 1h and the resulting derivatives of the mono-oxosteroids provided a 70-1600-fold higher sensitivity compared to intact steroids. However, HMP was unsuitable for di-oxosteroids, such as androstenedione and progesterone. The developed derivatization procedure was applied to the LC-ESI-MS analysis of 5alpha-dihydrotestosterone in human prostate, and allowed the reproducible quantification of nanogram/gram level of the androgen with a 10-mg sample.
Related Concept Videos
Carboxylic Acids to Methylesters: Alkylation using Diazomethane
Hydroboration-Oxidation of Alkenes
Oxidation of Alkenes: Syn Dihydroxylation with Osmium Tetraoxide
Diazonium Group Substitution: –OH and –H
