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Updated: Aug 15, 2026

Ammonia Fiber Expansion (AFEX) Pretreatment of Lignocellulosic Biomass
Published on: April 18, 2020
Short-term harmful effects of ammonia nitrogen on activated sludge microfauna
Jaume Puigagut1, Humbert Salvadó, Joan García
1Environmental Engineering Division, Department of Hydraulics, Coastal and Environmental Engineering, Technical University of Catalonia, c. Jordi Girona 1-3, Mòdul D-1, 08034 Barcelona, Spain. jaume.puigagut@upc.edu
Abstract:
The response of activated sludge microfauna in terms of abundance and diversity has been analysed to evaluate both the toxic effect of ammonia nitrogen and the acclimatisation capacity of these microorganisms to its toxicity. The harmful effect of ammonia nitrogen was studied by means of two toxicological tests. The ammonia concentrations tested were: 9, 20, 30 and 50mg NH4+-N/l in the first toxicological test and 30, 40, 50 and 80 mg NH4+-N/l in the second. The results suggest that ammonia nitrogen causes a clear but reversible toxic effect on microfauna abundance when its concentrations are around three times higher than that which the microfauna is used to. Chilodonella uncinata and Acineria uncinata were the ciliates least affected by the ammonia nitrogen toxicity. Furthermore, the majority of microfauna groups analysed (gymnamoebae and ciliates) showed capability for acclimatisation to ammonia nitrogen in terms of abundance.
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