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Methods to Assess Microbial Populations01:30

Methods to Assess Microbial Populations

Assessing microbial populations is crucial for understanding microbial roles in health, ecology, and industry. Various complementary techniques—both culture-based and molecular—enable detailed analysis of microbial abundance, diversity, and function.Viable Plate CountThe viable plate count is a traditional culture-based method used to estimate the number of living microbes in a sample. After serial dilution, the sample is spread onto nutrient agar plates. Each viable cell forms a visible...

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Experimental Protocol for Detecting Cyanobacteria in Liquid and Solid Samples with an Antibody Microarray Chip
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Published on: February 7, 2017

High-Throughput Screening of Cyanobacterial PHB Production Using Fluorescence-Based Detection.

Artai Lage Julià1, Beatriz Altamira-Algarra1, Joan Garcia1,2

  • 1GEMMA-Group of Environmental Engineering and Microbiology, Department of Civil and Environmental Engineering, Escola d'Enginyeria de Barcelona Est (EEBE), Universitat Politècnica de Catalunya·BarcelonaTech, Barcelona, Spain.

Microbial Biotechnology
|June 4, 2026
PubMed
Summary
This summary is machine-generated.

Cyanobacteria can produce polyhydroxybutyrate (PHB), a bioplastic. Optimal conditions for PHB production in Synechocystis and Leptolyngbya were identified and successfully scaled up in photobioreactors.

Keywords:
BG11 mediaNile bluePHBbiopolymercyanobacteria

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Last Updated: Jun 5, 2026

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Spectrophotometric Determination of Phycobiliprotein Content in Cyanobacterium Synechocystis
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Published on: September 11, 2018

Area of Science:

  • Microbiology
  • Biotechnology
  • Synthetic Biology

Background:

  • Cyanobacteria are photosynthetic microorganisms with potential for bioplastic production.
  • Polyhydroxybutyrate (PHB) is a biodegradable polymer synthesized by various bacteria, including cyanobacteria.
  • Efficient and scalable methods for PHB production are crucial for sustainable bioplastic manufacturing.

Purpose of the Study:

  • To screen and identify optimal conditions for polyhydroxybutyrate (PHB) production in different cyanobacterial strains.
  • To evaluate the potential of Synechocystis sp., Leptolyngbya sp., and a co-culture for PHB accumulation.
  • To validate the identified optimal conditions through scale-up experiments in photobioreactors.

Main Methods:

  • High-throughput screening using multi-well plates and Nile blue fluorescence for rapid PHB estimation.
  • Testing various conditions including nitrogen depletion, darkness, temperature, and acetate supplementation.
  • Scale-up validation in 1-L photobioreactors to assess PHB yield under optimized conditions.

Main Results:

  • Nile blue fluorescence enabled faster PHB estimation compared to gas chromatography.
  • Optimal conditions for PHB accumulation were identified as nitrogen depletion, darkness, 30°C, and acetate supplementation after glycogen accumulation.
  • Successful scale-up yielded 7% PHB dry cell weight (dcw) for Synechocystis sp. and 13% PHB dcw for Leptolyngbya sp.

Conclusions:

  • High-throughput screening is an effective method for optimizing PHB production conditions in cyanobacteria.
  • Leptolyngbya sp. shows significant potential for PHB accumulation.
  • A dedicated glycogen pre-accumulation step is not necessary for efficient PHB synthesis directly from acetate in these cyanobacteria.