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Updated: Aug 15, 2026

A Rapid Technique for the Visualization of Live Immobilized Yeast Cells
Published on: November 9, 2006
Use of a novel immobilization yeast system for winemaking
Rafael A Peinado1, Juan J Moreno, Oscar Maestre
1Department of Agricultural Chemistry, University of Cordoba, Campus Universitario de Rabanales, Eds 1C-3 and 2C-6, 14014, Córdoba, Spain.
Abstract:
Penicillium was used to immobilize Saccharomyces cerevisiae, without using physico-chemical external supports, to form yeast biocapsules. The biocapsules, once the Penicillium was killed by the ethanol produced, were used in a grape must fermentation. Must fermentation was carried out for 160 h with the biocapsules and for 300 h with free yeast cells. Acetaldehyde (84 vs. 63 mg/l), isobutanol (217 vs. 194 mg/l), L: -proline (7.7 vs. 6.5 mM: ) and aspartic acid (0.42 vs. 0 mM: ) in final wine were higher with the biocapsules than with free cells.
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