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Related Experiment Videos

Detecting single stranded DNA with a solid state nanopore.

Daniel Fologea1, Marc Gershow, Bradley Ledden

  • 1Department of Physics, University of Arkansas, Fayetteville, AR 72701, USA.

Nano Letters
|October 13, 2005
PubMed
Summary
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Voltage-biased solid-state nanopores detect single-stranded DNA at high pH. A significant fraction of events show constant charge deficit, indicating varied molecular configurations during translocation, advancing nanopore sequencing.

Area of Science:

  • Biophysics
  • Nanotechnology
  • Molecular Biology

Background:

  • Solid-state nanopores offer a promising platform for single-molecule analysis.
  • Characterizing DNA translocation dynamics is crucial for sequencing applications.

Purpose of the Study:

  • To investigate the translocation behavior of single-stranded DNA (ssDNA) through solid-state nanopores.
  • To determine if ssDNA adopts folded or unfolded configurations during nanopore passage.

Main Methods:

  • Utilizing voltage-biased solid-state nanopores.
  • Operating the nanopore detector at high pH (> 11.6).
  • Analyzing molecular event durations and blockade currents.

Main Results:

  • Observed a significant fraction of ssDNA translocation events exhibiting a constant event charge deficit (ecd).

Related Experiment Videos

  • This constant ecd suggests a distribution of folded and unfolded ssDNA configurations during passage.
  • A surprisingly large proportion of translocating ssDNA molecules were found to be unfolded.

    • Conclusions:

    • High pH operation of solid-state nanopores reveals complex ssDNA translocation dynamics.
    • The presence of unfolded ssDNA configurations is a key factor in nanopore signal interpretation.
    • These findings represent a significant advancement towards solid-state nanopore-based single-molecule sequencing.