Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Stem-loop oligonucleotides as tools for labelling double-stranded DNA.

Bénédicte Géron-Landre1, Thibaut Roulon, Christophe Escudé

  • 1Laboratoire Régulation et Dynamique des Génomes, Département Régulations, Développement et Diversité Moléculaire, Muséum National d'Histoire Naturelle, Paris Cedex, France.

The FEBS Journal
|October 13, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Epigenetic rewriting at centromeric DNA repeats leads to increased chromatin accessibility and chromosomal instability.

Epigenetics & chromatin·2021
Same author

MNHN-Tree-Tools: a toolbox for tree inference using multi-scale clustering of a set of sequences.

Bioinformatics (Oxford, England)·2021
Same author

The Targeted Sequencing of Alpha Satellite DNA in Cercopithecus pogonias Provides New Insight Into the Diversity and Dynamics of Centromeric Repeats in Old World Monkeys.

Genome biology and evolution·2018
Same author

A Ligand-Modulated Padlock Oligonucleotide for Supercoiled Plasmids.

Angewandte Chemie (International ed. in English)·2018
Same author

Interaction of fluorescently labeled pyrrole-imidazole polyamide probes with fixed and living murine and human cells.

Biochimie·2018
Same author

Diversity and distribution of alpha satellite DNA in the genome of an Old World monkey: Cercopithecus solatus.

BMC genomics·2016

We developed a DNA labeling method using stem-loop triplex forming oligonucleotides (TFOs) that encircle DNA targets. This technique allows sensitive detection of specific DNA sequences, forming stable topological complexes.

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Oligonucleotide Chemistry

Background:

  • Sequence-specific DNA recognition is crucial for molecular biology applications.
  • Triplex forming oligonucleotides (TFOs) offer a route for specific DNA binding.
  • Developing stable and sensitive DNA labeling strategies remains an active research area.

Purpose of the Study:

  • To report a novel DNA labeling strategy utilizing stem-loop TFOs.
  • To investigate the formation and properties of topological complexes between TFOs and DNA targets.
  • To assess the sensitivity and specificity of this labeling approach.

Main Methods:

  • Design and synthesis of stem-loop triplex forming oligonucleotides (TFOs).
  • Ligation of TFOs to DNA fragments to form topological complexes.

Related Experiment Videos

  • Investigation of factors influencing complex formation (e.g., TFO sequence, conditions).
  • Assessment of complex stability and detection sensitivity.

    • Main Results:

    • A sequence-specific DNA labeling strategy using stem-loop TFOs was established.
    • Topological complexes formed by TFOs encircling DNA targets.
    • Complex formation was efficient with GT or TC TFOs, enhanced by the stem-loop structure.
    • The TFO-DNA complexes remained associated even under non-hybridizing conditions.
    • Detection of a 20-bp target sequence at subfemtomolar levels was achieved.

    Conclusions:

    • Stem-loop TFOs can form stable topological complexes with specific DNA targets.
    • This method provides high specificity and sensitivity for DNA sequence detection.
    • The developed approach holds promise for creating new tools for labeling short DNA sequences.