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Updated: Aug 15, 2026
Development and Functional Characterization of Murine Tolerogenic Dendritic Cells
Published on: May 18, 2018
Attenuated alloreactivity of dendritic cells engineered with surface-modified microspheres carrying a plasmid
Liang Jia1, Jeffrey R Kovacs, Ying Zheng
1Division of Pharmaceutical Sciences, Duquesne University, 600 Forbes Ave., Mellon 413, Pittsburgh, PA, USA.
Abstract:
In the present study, we investigated MS(O10H6) as a carrier system to introduce a plasmid encoding murine interleukin-10 (pIL-10) to modulate alloreactivity of dendritic cells (DC). Results indicate that MS(O10H6) formed stable and protective nano-sized particles with pIL-10. Gene-modified DC elicited weak proliferation of allogeneic CD4 and CD8 T cells in vitro. Using cell-embedded Matrigel as a surrogate graft, we also showed that DC transfected with MS(O10H6) complexed with pIL-10 suppressed host cell infiltration in vivo. These data demonstrate that the self-assembled system of MS(O10H6) is an effectual delivery vehicle for plasmid-based modulation of DC-dependent allogeneic T cell responses.
